Coding
Part:BBa_K4165089:Design
Designed by: Mennatallah Mahmoud Mohamed Abdelzaher Turky Group: iGEM22_CU_Egypt (2022-09-30)
WAP-four disulfide core domain 2 serine protease inhibitor.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 115
Illegal PstI site found at 183 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 115
Illegal PstI site found at 183 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 115
Illegal PstI site found at 183 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 115
Illegal PstI site found at 183
Illegal NgoMIV site found at 3 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon optimized to be expressed in E.coli
Source
genomic sequence retrieved from the literature